一种用于检测DNA抗体的简单、快速酶联免疫吸附测定法。
A simple, rapid ELISA method for the detection of DNA antibodies.
作者信息
Stokes R P, Cordwell A, Thompson R A
出版信息
J Clin Pathol. 1982 May;35(5):566-73. doi: 10.1136/jcp.35.5.566.
Abstract
Employing an enzyme-linked immunosorbent assay (ELISA) technique the serum antibodies against native (double stranded) and denatured (single stranded) deoxyribonucleic acid (DNA) have been measured in various disease groups and a group of blood donor sera. The ELISA method has been compared with a radioimmunoassay method using native (double stranded) DNA is substrate antigen and a latex-fixation technique using particles coated with soluble deoxyribonucleoprotein (SNP). It is concluded that ELISA offers an economic and reliable alternative to isotope techniques for the assessment of antibody content in systemic lupus erythematosus (SLE) and related disease states for the clinical laboratory.
摘要
采用酶联免疫吸附测定（ELISA）技术，在不同疾病组和一组献血者血清中检测了针对天然（双链）和变性（单链）脱氧核糖核酸（DNA）的血清抗体。已将ELISA方法与以天然（双链）DNA为底物抗原的放射免疫测定方法以及使用包被有可溶性脱氧核糖核蛋白（SNP）的颗粒的乳胶固定技术进行了比较。得出的结论是，对于临床实验室评估系统性红斑狼疮（SLE）及相关疾病状态下的抗体含量而言，ELISA为同位素技术提供了一种经济且可靠的替代方法。
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