Cekan S Z
J Steroid Biochem. 1983 Jul;19(1B):403-5. doi: 10.1016/0022-4731(83)90194-2.
A valid steroid immunoassay is the one which correctly describes the contents in unknown samples. Such an assay has to be accurate (devoid of bias) and precise. Assay accuracy can be assessed by a comparison of the results with those obtained by a reference method, e.g. gas liquid chromatography-mass spectrometry. Alternatively, the accuracy assessment can be made in terms of the purity of the compound assayed. The test of parallelism or the test of radiochemical purity can be used for this purpose. The former test, however, is not fully reliable. The degree of precision is of great importance especially when validity of individual measurements is considered. A low reproducibility (a high between-assay variation) leads to results of uncertain validity. An increased standardization and simplification of assays may result in a substantial improvement of the precision, and thus validity.
有效的类固醇免疫测定法是能正确描述未知样品中成分的方法。这样的测定法必须准确（无偏差）且精密。测定法的准确性可通过将结果与参考方法（如气相色谱 - 质谱法）所获结果进行比较来评估。或者，准确性评估也可依据被测化合物的纯度进行。为此可使用平行性测试或放射化学纯度测试。然而，前一种测试并非完全可靠。精密度程度极为重要，尤其是在考虑单个测量的有效性时。低重现性（高批间变异）会导致有效性结果不确定。测定法的标准化和简化程度提高可能会大幅提升精密度，进而提高有效性。
