French Deborah
Department of Laboratory Medicine, University of California San Francisco, 185 Berry Street, Suite 290, San Francisco, CA 94107, USA.
Bioanalysis. 2016 Jun;8(11):1203-19. doi: 10.4155/bio-2015-0025. Epub 2016 May 24.
Steroid hormones are measured clinically to determine if a patient has a pathological process occurring in the adrenal gland, or other hormone responsive organs. They are very similar in structure making them analytically challenging to measure. Additionally, these hormones have vast concentration differences in human serum adding to the measurement complexity. GC-MS was the gold standard methodology used to measure steroid hormones clinically, followed by radioimmunoassay, but that was replaced by immunoassay due to ease of use. LC-MS/MS has now become a popular alternative owing to simplified sample preparation than for GC-MS and increased specificity and sensitivity over immunoassay. This review will discuss these methodologies and some new developments that could simplify and improve steroid hormone analysis in serum.
临床上检测类固醇激素以确定患者的肾上腺或其他激素反应器官是否发生病理过程。它们的结构非常相似，这使得其分析测量具有挑战性。此外，这些激素在人血清中的浓度差异很大，增加了测量的复杂性。气相色谱 - 质谱联用（GC-MS）是临床上用于测量类固醇激素的金标准方法，其次是放射免疫测定法，但由于使用方便，后者已被免疫测定法所取代。液相色谱 - 串联质谱联用（LC-MS/MS）现在已成为一种流行的替代方法，因为它比GC-MS的样品制备更简单，并且比免疫测定法具有更高的特异性和灵敏度。本文将讨论这些方法以及一些可能简化和改进血清中类固醇激素分析的新进展。
