Holmquist J, Imasoto Y, Bengtsson E, Olsen B, Stenkvist B
J Histochem Cytochem. 1976 Dec;24(12):1218-24. doi: 10.1177/24.12.63509.
As part of a study of cytologic automation, microspectrophotometric investigation of Papanicolaou-stained cervical cells was performed, using a Leitz MPV-II scanning photometer connected to a PDP 8/F minicomputer. It was shown that the selection of one single wavelength may result in difficulties in detecting boundries between background and cytoplasm and/or between cytoplasm nucleus. A set of two wavelengths, 530 nm and 570 nm, were found to be optimal for the image processing of these cells.
作为一项细胞学自动化研究的一部分，使用连接到PDP 8/F小型计算机的Leitz MPV-II扫描光度计，对巴氏染色的宫颈细胞进行了显微分光光度研究。结果表明，选择单一波长可能会在检测背景与细胞质之间和/或细胞质与细胞核之间的边界时遇到困难。发现一组两个波长，即530纳米和570纳米，对于这些细胞的图像处理是最佳的。
