用于分离辐射敏感突变体的纸片复制法。
Paper replication method for isolation of radiation-sensitive mutants.
作者信息
Tan S T, Maxcy R B, Thompson T L
出版信息
Appl Environ Microbiol. 1983 Jul;46(1):233-6. doi: 10.1128/aem.46.1.233-236.1983.
Abstract
A filter paper replication system particularly useful for isolation of radiation-sensitive mutants of pigmented bacteria was devised. The fidelity of replication was high. Adhesion between a paper disk and a properly dried master plate provided adequate contact pressure. The replicas arising from this technique constitute a convenient apparatus for general application in isolation of clones sensitive to a discriminating treatment.
摘要
设计了一种滤纸复制系统，该系统对于分离色素细菌的辐射敏感突变体特别有用。复制的保真度很高。纸盘与适当干燥的原平板之间的粘附提供了足够的接触压力。由该技术产生的复制品构成了一种方便的设备，可普遍应用于分离对鉴别处理敏感的克隆。
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