Tan S T, Maxcy R B
Appl Environ Microbiol. 1986 Jan;51(1):88-90. doi: 10.1128/aem.51.1.88-90.1986.
A simple method for detection of radiation-inducible radiation resistance was developed by irradiating aliquots (0.01 ml) of cell suspension on agar plates. Part of each experimental plate was subjected to an induction treatment, and subsequent radiation resistance was compared with that of untreated cells on the same plate. The UV radiation resistance of a Micrococcus sp. was increased approximately 1.6 times by an induction treatment. This simple procedure of irradiating cells in a "fixed" position on agar avoided washing, centrifugation, and cell enumeration required in traditional methods.
通过在琼脂平板上照射细胞悬液等分试样（0.01毫升），开发出一种检测辐射诱导的辐射抗性的简单方法。每个实验平板的一部分进行诱导处理，然后将后续的辐射抗性与同一平板上未处理的细胞的辐射抗性进行比较。一种微球菌属细菌的紫外线辐射抗性通过诱导处理提高了约1.6倍。这种在琼脂上“固定”位置照射细胞的简单程序避免了传统方法中所需的洗涤、离心和细胞计数。
