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具有乙醇和视黄醇活性的大鼠视网膜乙醇脱氢酶的纯化及部分特性分析

Purification and partial characterization of a rat retina alcohol dehydrogenase active with ethanol and retinol.

作者信息

Julià P, Farrés J, Parés X

出版信息

Biochem J. 1983 Aug 1;213(2):547-50. doi: 10.1042/bj2130547.

DOI:10.1042/bj2130547
PMID:6351845
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1152161/
Abstract

Homogeneous alcohol dehydrogenase (ADH) from rat retina was obtained by chromatography on DEAE-Sepharose and AMP-hexane-Sepharose. The enzyme is a dimer of Mr congruent to 80000 and oxidizes ethanol using NAD+ as a cofactor. Careful activity determinations demonstrate unambiguously that rat retina ADH is active with retinol as a substrate. This result opens the question about the role of retina ADH in the visual cycle.

摘要

通过在DEAE-琼脂糖和AMP-己烷-琼脂糖上进行色谱分离，从大鼠视网膜中获得了均一的乙醇脱氢酶（ADH）。该酶是一种分子量约为80000的二聚体，以NAD⁺作为辅因子氧化乙醇。仔细的活性测定明确表明，大鼠视网膜ADH以视黄醇作为底物时具有活性。这一结果引发了关于视网膜ADH在视觉循环中作用的问题。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd67/1152161/1f1f27997ad0/biochemj00347-0266-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd67/1152161/1f1f27997ad0/biochemj00347-0266-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd67/1152161/1f1f27997ad0/biochemj00347-0266-a.jpg

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本文引用的文献

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