Damsky C H, Richa J, Solter D, Knudsen K, Buck C A
Cell. 1983 Sep;34(2):455-66. doi: 10.1016/0092-8674(83)90379-3.
An antiserum against material shed into serum-free medium by MCF-7 human mammary carcinoma cells (anti-SFM II) disrupts cell-cell interactions in murine mammary tumor epithelial cells (MMTE). We now report purification of an 80 kd glycoprotein (GP80) from SFM of MCF-7 mammary carcinoma cells that blocks the activity of anti-SFM II. Anti-SFM II also inhibits compaction of eight-cell mouse embryos, and purified GP80 blocks this reaction. An antiserum against purified GP80 (anti-GP80) has all adhesion-disrupting activities displayed by anti-SFM II. It recognizes one band at 80 kd in SFM and a 120 kd band in detergent extracts of epithelial but not fibroblastic cells. In immunofluorescence studies it is restricted to sites of cell-cell interaction in cultured epithelial cells. Thus a cell surface glycoprotein of 120 kd, the medium form of which is approximately 80 kd, which is neither species nor tissue specific, is expressed at early stages of mammalian development and is found on epithelia.
一种针对MCF-7人乳腺癌细胞分泌到无血清培养基中的物质的抗血清（抗SFM II）会破坏小鼠乳腺肿瘤上皮细胞（MMTE）中的细胞间相互作用。我们现在报告从MCF-7乳腺癌细胞的无血清培养基中纯化出一种80kd糖蛋白（GP80），它能阻断抗SFM II的活性。抗SFM II还会抑制八细胞小鼠胚胎的紧密化，而纯化的GP80能阻断这一反应。一种针对纯化后的GP80的抗血清（抗GP80）具有抗SFM II所表现出的所有破坏黏附的活性。它能识别无血清培养基中80kd的一条带以及上皮细胞而非成纤维细胞的去污剂提取物中的120kd的一条带。在免疫荧光研究中，它局限于培养的上皮细胞中的细胞间相互作用位点。因此，一种120kd的细胞表面糖蛋白，其在培养基中的形式约为80kd，既无物种特异性也无组织特异性，在哺乳动物发育早期表达，且存在于上皮细胞上。
