Vestweber D, Kemler R
Exp Cell Res. 1984 May;152(1):169-78. doi: 10.1016/0014-4827(84)90241-6.
A rabbit antiserum against a purified embryonal carcinoma (EC) cell surface glycoprotein interferes with cell-cell interaction in mouse preimplantation embryos. The 123 kD glycoprotein seems not to be an integral membrane component. The reactivity pattern of the antiserum was studied by immunofluorescence on cryostat sections of post-implantation embryos and of adult tissues. During embryonic development positive reactions were found on all epithelial cells, irrespective of their germ layer origin. Epithelial cells of adult tissues--tongue, uterus, gut, kidney, trachea and liver--react with the antibodies. The results are compared with cell-adhesive molecules previously described on EC cells and preimplantation embryos.
一种针对纯化的胚胎癌（EC）细胞表面糖蛋白的兔抗血清会干扰小鼠植入前胚胎中的细胞间相互作用。这种123 kD的糖蛋白似乎不是完整的膜成分。通过免疫荧光法对植入后胚胎和成年组织的冰冻切片研究了抗血清的反应模式。在胚胎发育过程中，在所有上皮细胞上均发现阳性反应，无论其胚层来源如何。成年组织（舌、子宫、肠道、肾脏、气管和肝脏）的上皮细胞与抗体发生反应。将结果与先前在EC细胞和植入前胚胎上描述的细胞粘附分子进行了比较。
