Youssefnejadian E, Brodovcky H, Johnson M, Craft I
Prostaglandins. 1978 Feb;15(2):239-53. doi: 10.1016/0090-6980(78)90163-6.
A simple method is described for the determination of 13, 14-dihydro-15-keto-prostaglandin F2alpha (PGFM), using a highly specific antiserum raised in New Zealand rabbits. It involves extraction of human peripheral venous plasma with diethyl ether after addition of tritiated PGFM and HCl. Radioimmunoassay is performed on appropriate aliquots; after 2 hours or overnight equilibration the bound and free metabolite are separated using dextran-coated charcoal. The mean values +/- S.D. obtained are as follows: healthy males 32 +/- 16 pg/ml, females during follicular phase 48 +/- 18 pg/ml, luteal phase 37 +/- 8 pg/ml, first trimester of pregnancy 66 +/- 33 pg/ml, second trimester 67 +/- 42 pg/ml and third trimester 72 +/- 26 pg/ml.
本文描述了一种使用在新西兰兔体内产生的高特异性抗血清来测定13,14-二氢-15-酮-前列腺素F2α（PGFM）的简单方法。该方法包括在加入氚标记的PGFM和盐酸后，用乙醚提取人外周静脉血浆。对适当的等分试样进行放射免疫测定；在2小时或过夜平衡后，使用葡聚糖包被的活性炭分离结合型和游离型代谢物。获得的平均值±标准差如下：健康男性为32±16 pg/ml，卵泡期女性为48±18 pg/ml，黄体期为37±8 pg/ml，妊娠早期为66±33 pg/ml，妊娠中期为67±42 pg/ml，妊娠晚期为72±26 pg/ml。
