Mitchell M D, Ebenhack K, Kraemer D L, Cox K, Cutrer S, Strickland D M
Prostaglandins Leukot Med. 1982 Nov;9(5):549-57. doi: 10.1016/0262-1746(82)90036-1.
A radioimmunoassay for 11-deoxy-13, 14-dihydro-15-keto-11, 16-cycloprostaglandin E2 (PGEM-II) is described. At pH 10.5, in the presence of albumin, 13, 14-dihydro-15-keto-prostaglandin E2 (PGEM-I) is transformed quantitatively into PGEM-II. Hence, after plasma samples are subjected to this transformation procedure, PGEM-II can be measured in such samples and be a true reflection of PGEM-I production and thereby prostaglandin E2 (PGE2) biosynthesis. The antiserum raised against PGEM-II is minimally cross-reactive with all prostaglandins tested (less than 0.01%). The mean least detectable mass of PGEM-II is 0.8 pg and the mean mass of added PGEM-II required to displace zero-point binding by 50% is 16.6 pg. The assay meets all the standard criteria for accuracy, reproducibility and parallelism. Concentrations of PGEM-II measured by this assay in peripheral plasma from men and nonpregnant women are similar to those reported for PGEM-I after measurements by gas chromatography - mass spectrometry. Plasma concentrations of PGEM-II in men were greater than in nonpregnant women. PGEM-II in increased concentration was found in early pregnancy although concentrations declined in the third trimester until labor when a significant increase was found. Concentrations of PGEM-II in umbilical venous plasma were significantly greater than those in maternal plasma.
本文描述了一种用于检测11-脱氧-13,14-二氢-15-酮-11,16-环前列腺素E2（PGEM-II）的放射免疫分析方法。在pH 10.5且存在白蛋白的条件下，13,14-二氢-15-酮前列腺素E2（PGEM-I）可定量转化为PGEM-II。因此，血浆样本经过此转化程序后，即可检测其中的PGEM-II，其可真实反映PGEM-I的生成情况，进而反映前列腺素E2（PGE2）的生物合成。针对PGEM-II产生的抗血清与所有检测的前列腺素的交叉反应极小（小于0.01%）。PGEM-II的平均最低检测质量为0.8 pg，使零点结合位移50%所需添加的PGEM-II的平均质量为16.6 pg。该分析方法符合准确性、重复性和平行性的所有标准准则。通过此分析方法测得的男性和未孕女性外周血中PGEM-II的浓度，与气相色谱-质谱法测定PGEM-I后报告的浓度相似。男性血浆中PGEM-II的浓度高于未孕女性。在妊娠早期发现PGEM-II浓度升高，尽管在妊娠晚期直至分娩时浓度下降，但在分娩时发现有显著升高。脐静脉血浆中PGEM-II的浓度明显高于母体血浆中的浓度。
