Scher W, Scher B M, Waxman S
Exp Hematol. 1983 Jul;11(6):490-8.
Proteases stimulate mouse erythroleukemia (MEL) cell differentiation and multiplication. The stimulation of differentiation is synergistically increased by low concentrations of dimethyl sulfoxide. Synergism of other low molecular weight inducers with representative proteases, alpha-chymotrypsin and protease V8, was tested. Hemin. hypoxanthine, actinomycin D, aminonucleoside of puromycin, hexamethylene bisacetamide, and 5-azacytidine were also found to act synergistically with this protease to augment MEL cell hemoglobin production, but not cell multiplication. Fatty acids (acetate, propionate, butyrate, isobutyrate, and valerate), prostaglandins A1 and E1, amino acids, and amino acid analogs and metabolites did not act synergistically with chymotrypsin. Some physiologic amino acids were found to be weak inducers. Several of the low molecular weight inducers also acted synergistically with protease V8 in inducing differentiation, and, as with chymotrypsin, did not act synergistically in stimulating cell multiplication. Like chymotrypsin, protease V8 did not act synergistically with butyrate. The earlier finding that proteases, but not low molecular weight inducers, stimulate cell multiplication during the induction of differentiation was confirmed. Carboxypeptidase A also was found to be an inducer.
蛋白酶可刺激小鼠红白血病（MEL）细胞的分化和增殖。低浓度的二甲基亚砜可协同增强分化的刺激作用。测试了其他低分子量诱导剂与代表性蛋白酶α-胰凝乳蛋白酶和蛋白酶V8的协同作用。还发现血红素、次黄嘌呤、放线菌素D、嘌呤霉素氨基核苷、六甲撑双乙酰胺和5-氮杂胞苷也能与这种蛋白酶协同作用，增加MEL细胞血红蛋白的产生，但不促进细胞增殖。脂肪酸（乙酸盐、丙酸盐、丁酸盐、异丁酸盐和戊酸盐）、前列腺素A1和E1、氨基酸以及氨基酸类似物和代谢物与胰凝乳蛋白酶没有协同作用。发现一些生理性氨基酸是弱诱导剂。几种低分子量诱导剂在诱导分化方面也与蛋白酶V8协同作用，并且与胰凝乳蛋白酶一样，在刺激细胞增殖方面没有协同作用。与胰凝乳蛋白酶一样，蛋白酶V8与丁酸盐没有协同作用。先前关于蛋白酶而非低分子量诱导剂在分化诱导过程中刺激细胞增殖的发现得到了证实。还发现羧肽酶A是一种诱导剂。
