用单克隆抗体对单细胞标本中的抗原进行酶免疫细胞化学同步检测。
Simultaneous enzyme-immunocytochemical detection of antigens in monocellular specimens with monoclonal antibodies.
作者信息
Feller A C, Parwaresch M R
出版信息
J Immunol Methods. 1983 Oct 14;63(2):273-9. doi: 10.1016/0022-1759(83)90431-3.
An immunoperoxidase and immunoalkaline phosphatase technique is described specially devised for separate cell specimens such as smears, cytospins, and tissue imprints. The reaction may be used alone or in combination for simultaneous staining with 2 different monoclonal antibodies. Monoclonal antibodies Leu3a, Leu2a, T4, T8, anti-kappa, and anti-lambda light chain were used in combination to visualize subsets of human lymphocytes selectively. Six cases of chronic lymphocytic leukemia were classified by this method.
本文描述了一种免疫过氧化物酶和免疫碱性磷酸酶技术，该技术是专门为涂片、细胞离心涂片和组织印片等单独的细胞标本设计的。该反应可单独使用，也可与两种不同的单克隆抗体联合用于同时染色。联合使用单克隆抗体Leu3a、Leu2a、T4、T8、抗κ和抗λ轻链来选择性地观察人类淋巴细胞亚群。用该方法对6例慢性淋巴细胞白血病进行了分类。
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