[多形汉逊酵母非产孢菌株通过原生质体融合进行杂交]
[Hybridization of asporogenic strains of Hansenula polymorpha yeasts by protoplast fusion].
作者信息
Savchenko G V, Kapul'tsevich Iu G, Temina A V, Nikitina I A
出版信息
Mikrobiologiia. 1983 May-Jun;52(3):449-54.
PMID:6353182
Abstract
Intrastrain and interstrain hybrids of different ploidy were produced by the fusion of protoplasts of Hansenula polymorpha haploid and diploid strains. The diploid hybrids were found to be stable in contrast to the triploid and tetraploid hybrids. The instability of the triploid and tetraploid states in Hansenula polymorpha was expressed in the elevated frequency of spontaneous formation of auxotrophic markers and in the decreased content of DNA per cell in the course of storage.
摘要
通过多形汉逊酵母单倍体和二倍体菌株原生质体融合，产生了不同倍性的菌株内和菌株间杂种。与三倍体和四倍体杂种相比，二倍体杂种被发现是稳定的。多形汉逊酵母中三倍体和四倍体状态的不稳定性表现为自发形成营养缺陷型标记的频率升高，以及在储存过程中每个细胞的DNA含量降低。
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