Polotskiĭ A E, Frenkel' S Ia
Mol Biol (Mosk). 1983 Jul-Aug;17(4):803-8.
The sedimentation-biochemical method of determination of enzymes' sedimentation coefficients and estimation of their molecular masses based on the Tiselius' transport technique is discussed. The method permits to determine the sedimentation coefficients of enzyme in the starting mixtures without their preliminary purification. The use of different substrats permits to carry out the studying of several enzymes simultaneously. The frameworks of the method are discussed.
讨论了基于Tiselius迁移技术的沉降生化法测定酶的沉降系数并估算其分子量。该方法无需对酶进行初步纯化即可测定起始混合物中酶的沉降系数。使用不同的底物可以同时对几种酶进行研究。还讨论了该方法的框架。
