Mosinger B, Kotoul P, Michl J
Physiol Bohemoslov. 1983;32(4):352-8.
Insulin (5 micrograms/ml), thyroxine (1 microgram/ml) and beta-oestradiol (0.1 microgram/ml), if added separately to the medium, did not significantly influence the growth of normal human diploid LEP 19 cells cultivated in Eagle's MEM medium with 1% foetal calf serum. Combined, they did not influence the growth of LEP 19 cells in serum-free MEM medium either. Under the same cultivation conditions, insulin caused 37%, thyroxine 24% and a mixture of insulin, thyroxine and beta-oestradiol 325% (without serum) or 15% (with 1% foetal calf serum) stimulation of the growth of human heteroploid HeLa cells. Isolated beta-oestradiol significantly inhibited the growth of HeLa cells.
胰岛素（5微克/毫升）、甲状腺素（1微克/毫升）和β-雌二醇（0.1微克/毫升），若分别添加到培养基中，对在含1%胎牛血清的伊格尔氏最低必需培养基中培养的正常人二倍体LEP 19细胞的生长没有显著影响。将它们组合起来，对无血清最低必需培养基中LEP 19细胞的生长也没有影响。在相同培养条件下，胰岛素对人异倍体海拉细胞的生长有37%的促进作用，甲状腺素有24%的促进作用，胰岛素、甲状腺素和β-雌二醇的混合物在无血清时对海拉细胞生长有325%的促进作用，在含1%胎牛血清时则有15%的促进作用。单独的β-雌二醇显著抑制海拉细胞的生长。
