[梅毒治疗前后不同阶段FTA abs IgM的定量测定]
[Quantitative determination of FTA abs IgM in different stages of syphilis before and after treatment].
作者信息
Lefèvre J C, Prère M F, Abbal M, Lareng M B
出版信息
Ann Dermatol Venereol. 1983;110(5):425-30.
The titres of treponemal specific IgM and IgG antibodies were determined by monospecific immunofluorescence (FTA abs IgM/IgG) on 191 sera from 107 patients with treated or untreated syphilis, at various stages and from 10 neonates born from seropositive mothers. IgM antitreponemal antibody was found in all cases of untreated syphilis but two neurosyphilis. After treatment the antibody usually disappeared within one year. However it persisted in three patients treated two years ago or more. When sera from these patients were fractionated by density gradient ultracentrifugation and examined for antitreponemal antibodies of the IgM classe (FTA abs IgM 19S) they never showed detectable concentrations of IgM. False reactive results as false non reactivity due to competitive inhibition, observed with FTA abs IgM can be eliminated after separation of immunoglobulins.
采用单特异性免疫荧光法（FTA abs IgM/IgG）检测了107例处于不同阶段、梅毒已治疗或未治疗患者的191份血清以及10例血清反应阳性母亲所生新生儿的血清中梅毒螺旋体特异性IgM和IgG抗体的滴度。除两例神经梅毒外，所有未经治疗的梅毒患者均检测到IgM抗梅毒螺旋体抗体。治疗后，该抗体通常在一年内消失。然而，在两年或更久之前接受治疗的三名患者中，该抗体仍然存在。当对这些患者的血清进行密度梯度超速离心分离并检测IgM类抗梅毒螺旋体抗体（FTA abs IgM 19S）时，从未检测到可检测浓度的IgM。通过免疫球蛋白分离，可以消除FTA abs IgM观察到的由于竞争性抑制导致的假阳性反应结果和假阴性反应结果。
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