Vântu A, Voiculet N, Ivănescu M, Balaban C
Med Interne. 1978 Jan-Mar;16(1):61-6.
To demonstrate the presence of specific antigens in the normal and malignant gastric juice the immunological and chemical features of certain antigens with glycoprotein structure from malignant gastric jucice and gastric tumors were studied comparatively with those of antigens obtained from normal gastric juice and gastric tissue. The investigation was carried out in tumor tissue extracts from 7 patients with malignant gastric tumors and in gastric juice from 45 patients with other non-cancerous diseases. From the five fractions obtained, which gave immunoelectrophoretic precipitin lines in the beta and alpha-globulin regions with the antiserum against total normal gastric juice, a single specific was separated by means of successive chromatographies on sephadex G--100 and DEAE-Sephadex -a--50 (eluted in NaCl concentration gradient). This fraction represented 11.1 per cent of the total proteins. None of the five fractions could be identified with carcinoembryonic antigen (value in gastric juice and gastric malignant tumors = 2 to 10 ng per mg lyophilized products).
为了证明正常和恶性胃液中特定抗原的存在，对来自恶性胃液和胃肿瘤的某些具有糖蛋白结构的抗原的免疫学和化学特性，与从正常胃液和胃组织中获得的抗原进行了比较研究。研究在7例胃恶性肿瘤患者的肿瘤组织提取物和45例患有其他非癌性疾病患者的胃液中进行。从获得的五个组分中，用抗全正常胃液抗血清在β和α球蛋白区域产生免疫电泳沉淀线，通过在葡聚糖凝胶G - 100和二乙氨基乙基葡聚糖 - a - 50上连续色谱法（在NaCl浓度梯度中洗脱）分离出单一特异性物质。该组分占总蛋白的11.1%。五个组分中没有一个能与癌胚抗原鉴定一致（胃液和胃恶性肿瘤中的值 = 每毫克冻干产物2至10纳克）。
