一种在微波辐照后解剖大鼠脑离散区域的方法。

A method for dissection of discrete regions of rat brain following microwave irradiation.

作者信息

O'Callaghan J P, Lavin K L, Chess Q, Clouet D H

出版信息

Brain Res Bull. 1983 Jul;11(1):31-42. doi: 10.1016/0361-9230(83)90054-0.

Abstract

A simple microdissection technique for obtaining discrete areas from rat brains exposed to microwave irradiation is described and illustrated. Using the atlas of Pellegrino et al. [10] as a guide, stereotaxically defined areas were removed from coronal sections prepared with sectioning stages constructed from microscope slides. The dissection of sixteen discrete regions is shown in photographic and schematic form. This technique may prove useful for examining neurochemical processes in discrete areas of the rat central nervous system and may aid in establishing the distribution of pharmacological and toxicological agents at a neuroanatomical level.

摘要

本文描述并展示了一种简单的显微解剖技术，用于从暴露于微波辐射的大鼠大脑中获取离散区域。以佩莱格里诺等人[10]的图谱为指导，从用显微镜载玻片构建的切片载物台上制备的冠状切片中，立体定位确定的区域被移除。十六个离散区域的解剖以照片和示意图的形式展示。该技术可能被证明对检查大鼠中枢神经系统离散区域的神经化学过程有用，并可能有助于在神经解剖学水平上确定药理和毒理制剂的分布。