Laszlo A, Bissell M J
Exp Cell Res. 1983 Oct;148(1):221-34. doi: 10.1016/0014-4827(83)90201-x.
We have found an increased rate of vimentin synthesis in TPA-treated chicken embryonic fibroblasts, as shown by two-dimensional electrophoretic separation of newly synthesized polypeptides. The capacity of TPA to elicit this effect correlated with its activity as a tumor promoter. Treatment with TPA also altered the organization of the vimentin-containing intermediate filaments, as revealed by immunofluorescence. Treatments which inhibited the TPA-induced rearrangements of vimentin filaments did not prevent the increase in the rate of vimentin synthesis, indicating that gross alterations in cytoskeletal organization were not the immediate cause of the altered vimentin synthesis. On the other hand, alterations in the rate of vimentin synthesis may be a necessary, but not sufficient condition for alterations in vimentin filament organization. TPA as a positive modulator of vimentin synthesis may serve as a useful probe for an eventual understanding of the dynamics of the mechanisms that control the assembly and organization of vimentin filaments.
我们发现，经佛波酯（TPA）处理的鸡胚成纤维细胞中波形蛋白的合成速率增加，这一点通过对新合成多肽进行二维电泳分离得以证明。TPA引发这种效应的能力与其作为肿瘤促进剂的活性相关。免疫荧光显示，用TPA处理还改变了含波形蛋白的中间丝的组织形式。抑制TPA诱导的波形蛋白丝重排的处理并未阻止波形蛋白合成速率的增加，这表明细胞骨架组织的总体改变并非波形蛋白合成改变的直接原因。另一方面，波形蛋白合成速率的改变可能是波形蛋白丝组织改变的必要条件，但并非充分条件。TPA作为波形蛋白合成的正向调节剂，可能成为最终理解控制波形蛋白丝组装和组织机制动态变化的有用探针。
