Vincenzini M T, Favilli F, Treves C, Vanni P, Baccari V
Int J Biochem. 1983;15(10):1283-6. doi: 10.1016/0020-711x(83)90220-3.
A study has been carried out in order to explain the enzyme-palmitoleate interaction. The highly purified and crystalline enzymes representative of fundamental metabolic pathways were: alcohol dehydrogenase (ADH), lactate dehydrogenase (LDH), malate dehydrogenase (MDH), isocitrate dehydrogenase (ICDH), glucose-6-phosphate dehydrogenase (G6P-DH), alkaline phosphatase. The enzyme-palmitoleate interaction was studied as a phenomenon time-independent (inhibition) and time-dependent (inactivation). Palmitoleate inhibited remarkably LDH, MDH, ICDH and G6P-DH. A kinetic analysis of the inhibitory action of palmitoleate on LDH and MDH was also carried out. Inactivation studies have shown that ADH and alkaline phosphatase are not sensitive to palmitoleate action, unlike the other enzymes. A comparison was made between the action of palmitoleate and that of a synthetic anionic detergent, sodium dodecyl sulfate (SDS).
为了解释酶与棕榈油酸酯的相互作用，开展了一项研究。代表基本代谢途径的高度纯化的结晶酶有：乙醇脱氢酶（ADH）、乳酸脱氢酶（LDH）、苹果酸脱氢酶（MDH）、异柠檬酸脱氢酶（ICDH）、葡萄糖-6-磷酸脱氢酶（G6P-DH）、碱性磷酸酶。酶与棕榈油酸酯的相互作用被作为一种与时间无关（抑制）和与时间有关（失活）的现象来研究。棕榈油酸酯显著抑制LDH、MDH、ICDH和G6P-DH。还对棕榈油酸酯对LDH和MDH的抑制作用进行了动力学分析。失活研究表明，与其他酶不同，ADH和碱性磷酸酶对棕榈油酸酯的作用不敏感。对棕榈油酸酯的作用与合成阴离子洗涤剂十二烷基硫酸钠（SDS）的作用进行了比较。
