Schauer R, Reuter G, Mühlpfordt H, Andrade A F, Pereira M E
Hoppe Seylers Z Physiol Chem. 1983 Aug;364(8):1053-7. doi: 10.1515/bchm2.1983.364.2.1053.
Different strain of Trypanosoma cruzi were analysed to have 65--105 micrograms of sialic acids per 10(10) cells. By thin-layer chromatography, and in part by gas liquid chromatography and gas-liquid chromatography-mass spectrometry, all strains were found to contain N-acetyl- and N-glycoloylneuraminic acid in various ratios. After incubation of the parasites with either [3H]acetate or N-acetyl-[3H]mannosamine, no radioactivity was found in the sialic acids, thus leading to the suggestion that the parasites are unable to synthesize sialic acids from their precursors.
对不同菌株的克氏锥虫进行分析，发现每10¹⁰个细胞含有65 - 105微克唾液酸。通过薄层色谱法，部分通过气相色谱法和气相色谱 - 质谱联用分析法，发现所有菌株均含有不同比例的N - 乙酰神经氨酸和N - 羟乙酰神经氨酸。在用[³H]乙酸盐或N - 乙酰 - [³H]甘露糖胺培养寄生虫后，在唾液酸中未发现放射性，因此表明寄生虫无法从其前体合成唾液酸。
