Jones D E, Kanarek K S, Angel J L, Lim D V
J Clin Microbiol. 1983 Sep;18(3):526-8. doi: 10.1128/jcm.18.3.526-528.1983.
Strong multiple reactions often occur with the Phadebact Streptococcus test when the culture contains blood. These reactions interfere with the identification of the Lancefield groups of streptococci. Group B streptococci from the vagina of pregnant women are difficult to identify by slide coagglutination because of the frequent presence of blood on culture swabs. Elimination of these multiple reactions caused by blood would permit rapid identification of group B streptococci in pregnant women. Vaginal broth cultures were examined to determine the cause of multiple reactions with slide coagglutination and to eliminate them from the testing procedure. Of 245 maternal broth cultures, 135 (55%) yielded multiple reactions when tested by coagglutination. Such reactions were either eliminated or greatly diminished by heating the broth sample to 90 degrees C for 10 min. It was also found that globulins in the serum may be responsible for multiple reactions with blood. This heating protocol will permit vaginal broth cultures to be rapidly tested for group B streptococci by slide coagglutination.
当培养物中含有血液时，Phadebact链球菌试验常常会出现强烈的多重反应。这些反应会干扰链球菌兰斯菲尔德分组的鉴定。由于培养拭子上经常有血液存在，孕妇阴道中的B组链球菌很难通过玻片凝集试验进行鉴定。消除由血液引起的这些多重反应将有助于快速鉴定孕妇中的B组链球菌。对阴道肉汤培养物进行了检查，以确定玻片凝集试验出现多重反应的原因，并从检测程序中消除这些反应。在245份母体肉汤培养物中，135份（55%）通过凝集试验检测时产生了多重反应。将肉汤样品加热至90摄氏度10分钟后，此类反应要么被消除，要么大大减弱。还发现血清中的球蛋白可能是导致与血液发生多重反应的原因。这种加热方案将允许通过玻片凝集试验对阴道肉汤培养物快速检测B组链球菌。
