在托德-休伊特肉汤中评估黏菌素和萘啶酸用于选择性分离B组链球菌的效果。
Evaluation of colistin and nalidixic acid in Todd-Hewitt broth for selective isolation of group B streptocci.
作者信息
Fenton L J, Harper M H
出版信息
J Clin Microbiol. 1979 Feb;9(2):167-9. doi: 10.1128/jcm.9.2.167-169.1979.
Abstract
Todd-Hewitt broth (THB) containing colistin and nalidixic acid was compared with four other media with respect to efficacy of isolation of group B streptocci. It was effective as plain THB, THB with gentamicin and nalidixic acid and blood, and THB with colistin, nalidixic acid, and blood. THB with gentamicin and nalidixic acid, but without blood, was inhibitory to group B streptocci. The value of THB with colistin and nalidixic acid lies in its ability to successfully inhibit gram-negative organisms and still promote group B streptococcal growth without the addition of blood. This greatly reduces the time and expense of media preparation and permits early determination of bacterial growth, so that other means of rapidly identifying group B streptocci can be applied at the earliest possible time.
摘要
将含有黏菌素和萘啶酸的托德-休伊特肉汤（THB）与其他四种培养基在B族链球菌分离效果方面进行了比较。它与普通THB、含庆大霉素、萘啶酸和血液的THB以及含黏菌素、萘啶酸和血液的THB一样有效。含庆大霉素和萘啶酸但不含血液的THB对B族链球菌有抑制作用。含黏菌素和萘啶酸的THB的价值在于其能够成功抑制革兰氏阴性菌，并且在不添加血液的情况下仍能促进B族链球菌生长。这大大减少了培养基制备的时间和费用，并允许早期确定细菌生长情况，以便尽早应用其他快速鉴定B族链球菌的方法。
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