Lim D V, Smith R D, Day S
Can J Microbiol. 1979 Jan;25(1):40-3. doi: 10.1139/m79-006.
Grouping of beta-hemolytic streptococci was performed with the Phadebact Streptococcus Test, a coagglutination method, and the results compared with serological grouping by the standard Lancefield precipitin method. Of 171 clinical specimens examined, 169 (98.8%) were grouped correctly by the Phadebact Test after 24 h of continuous growth in Todd-Hewitt broth. In a parallel study, 96.9% of specimens that grew after only 4 h of incubation in broth were grouped correctly by the coagglutination method. In both studies, the accuracy of the coagglutination test was increased significantly by elimination of multiple-agglutination reactions through centrifugation of cultures and utilization of the supernatant fluid in the Phadebact Test.
采用凝固法Phadebact链球菌试验对β-溶血性链球菌进行分组，并将结果与标准的兰斯菲尔德沉淀法血清学分组结果进行比较。在171份检测的临床标本中，169份（98.8%）在托德-休伊特肉汤中连续培养24小时后通过Phadebact试验正确分组。在一项平行研究中，96.9%在肉汤中仅培养4小时后生长的标本通过凝固法正确分组。在两项研究中，通过对培养物进行离心以消除多重凝集反应，并在Phadebact试验中使用上清液，凝固试验的准确性显著提高。
