大鼠血管壁中的免疫反应性胰高血糖素。

Immunoreactive glucagon in the vascular walls of the rat.

作者信息

Tanaka J, Shiosaka S, Tsubouchi H, Gohda E, Daikuhara Y, Tohyama M

出版信息

Life Sci. 1983 Oct 17;33(16):1599-604. doi: 10.1016/0024-3205(83)90701-4.

Abstract

We detected immunohistochemically immunoreactive glucagon (IRG) in the smooth muscle of blood vessels and the myoepithelial cell of sweat glands of rats using two antisera against pancreatic glucagon; OAL-123 and 30K. The content of IRG in the blood vessels was found to be 320-1, 270 pg per g wet tissue weight. Filtration of the extracted IRG through a Bio Gel P-30 column yielded a single peak of IRG at 3,500 daltons, the same elution volume of pancreatic glucagon. These findings suggest that the blood vessels of the rats is one of the extrapancreatic sources of IRG in plasma, although physiological role of the IRG is not known.

摘要

我们使用两种抗胰高血糖素抗血清（OAL - 123和30K），通过免疫组织化学方法在大鼠血管平滑肌和汗腺肌上皮细胞中检测到免疫反应性胰高血糖素（IRG）。发现血管中IRG的含量为每克湿组织重量320 - 1270皮克。将提取的IRG通过Bio Gel P - 30柱过滤后，在3500道尔顿处得到一个单一的IRG峰，与胰高血糖素的洗脱体积相同。这些发现表明，大鼠的血管是血浆中IRG的胰腺外来源之一，尽管IRG的生理作用尚不清楚。