Cassone A, Scaringi L, Pesce C D, Titti F, Bistoni F, Marconi P, Rossi G B
Microbiologica. 1983 Jul;6(3):207-20.
Inactivated cellular preparations and cell wall materials of Candida albicans (CA) were tested for their capacity to suppress the growth of Friend Leukemia Cell (FLC)-induced tumours and the infection by Friend Leukemia Virus (FLV) in histocompatible mice. Factors affecting the inhibition of tumor growth by CA cellular preparations were: i) the schedule of agent administration; ii) the method of cell inactivation; iii) the FLC load. In particular, mice given 10(7) yeast cells, inactivated by cold alkali, on days -14 and +1 with respect to 10(4) FLC challenge on day 0 did not develop tumors. A crude cell wall fraction derived from cells extracted with hot alkali was still effective in reducing (but not suppressing) tumour growth whereas a purified, particulate glucan fraction (glucan "ghosts", essentially consisting of beta 1,3-1,6 glucan) was ineffective. No cellular preparation or cell wall fraction exerted anti-FLV effects (as shown by splenomegaly measurements) nor did any CA material induce interferon-like activity in the serum of animals injected with either FLC or FLV. Therefore, the observed antitumor activity by CA was not mediated by antiviral effects but possibly due to an "adjuvant-type", nonspecific, immunopotentiation of host antitumor response, as documented in other animal tumor models.
对白色念珠菌（CA）的灭活细胞制剂和细胞壁材料进行了测试，以评估它们抑制组织相容性小鼠中弗氏白血病细胞（FLC）诱导的肿瘤生长以及弗氏白血病病毒（FLV）感染的能力。影响CA细胞制剂抑制肿瘤生长的因素有：i）药剂给药方案；ii）细胞灭活方法；iii）FLC负荷。特别是，在相对于第0天10⁴个FLC攻击的第-14天和第+1天给予经冷碱灭活的10⁷个酵母细胞的小鼠未发生肿瘤。用热碱提取的细胞衍生的粗细胞壁级分在减少（但不是抑制）肿瘤生长方面仍然有效，而纯化的颗粒状葡聚糖级分（葡聚糖“幽灵”，主要由β1,3 - 1,6葡聚糖组成）无效。没有细胞制剂或细胞壁级分发挥抗FLV作用（通过脾肿大测量显示），也没有任何CA材料在注射FLC或FLV的动物血清中诱导干扰素样活性。因此，观察到的CA的抗肿瘤活性不是由抗病毒作用介导的，而是可能由于宿主抗肿瘤反应的“佐剂型”、非特异性免疫增强，正如在其他动物肿瘤模型中所记录的那样。
