Zavriev S K, Vorob'ev S M
Mol Biol (Mosk). 1983 Sep-Oct;17(5):1048-59.
Further research on bacteriophage T7 DNA penetration mechanism into E. coli cells during the infection was carried out. The DNA-RNA-hybridization on nitrocellulose filters revealed that in the presence of chloramphenicol the T7 DNA penetration from the virion into a host cell was coupled with its transcription by the bacterial RNA polymerase. The data obtained indicate that in the absence of antibiotics the penetration of a part of T7 genome which correspondes to class II and III genes is coupled with its transcription by a phage-specific RNA polymerase. Along with this the host restriction-modification system when its activity is not inhibited by the phage-induced proteins will be able to cleave the penetrated T7 DNA just after its transcription was accomplished. Considering these data along with our conception on direct involvement of transcription in T7 DNA penetration process during the infection one can suggest that E. coli RNA polymerase molecules which provide the phage DNA transport, are localized at the inner surface of cytoplasmic membrane.
针对噬菌体T7感染期间DNA进入大肠杆菌细胞的机制展开了进一步研究。硝酸纤维素滤膜上的DNA-RNA杂交实验表明，在氯霉素存在的情况下，T7 DNA从病毒粒子进入宿主细胞的过程与其由细菌RNA聚合酶进行的转录相偶联。所获数据表明，在无抗生素时，T7基因组中与II类和III类基因相对应的一部分的穿透过程与其由噬菌体特异性RNA聚合酶进行的转录相偶联。与此同时，宿主限制修饰系统在其活性未被噬菌体诱导蛋白抑制时，能够在穿透的T7 DNA完成转录后立即将其切割。结合这些数据以及我们关于转录在感染期间T7 DNA穿透过程中直接参与的观点，可以推测，负责噬菌体DNA转运的大肠杆菌RNA聚合酶分子定位于细胞质膜的内表面。
