Zavriev S K, Shemiakin M F
Mol Biol (Mosk). 1980 Nov-Dec;14(6):1387-91.
The cell-attached T7 phage DNA has been analyzed when E. coli was infected in the presence of chloramphenicol or rifampicin followed by sonication to provide phage desorption. Sucrose gradient sedimentation of the cell T7-DNA followed by HpaI digestion and agarose gel electrophoresis were performed. The results obtained suggest a gradual entrance of the T7-DNA molecule into the host cell starting on its left end bearing early genes. These data support the conception that the T7-DNA entrance into host cell is directly coupled with its transcription by RNA polymerase. At the same time one more HpaI fragment was found even in the cells, infected with the phage in the process of rifampicin. It may be that this fragment corresponds to the right end of the T7 chromosome, thus suggesting that short fragment of the T7-DNA right end can also enter the host cell early in infection.
当在氯霉素或利福平存在的情况下感染大肠杆菌，随后通过超声处理使噬菌体解吸附时，对细胞附着的T7噬菌体DNA进行了分析。对细胞T7-DNA进行蔗糖梯度沉降，然后进行HpaI消化和琼脂糖凝胶电泳。获得的结果表明，T7-DNA分子从携带早期基因的左端开始逐渐进入宿主细胞。这些数据支持了T7-DNA进入宿主细胞与其由RNA聚合酶转录直接相关的概念。同时，即使在利福平处理过程中感染噬菌体的细胞中也发现了另一个HpaI片段。可能这个片段对应于T7染色体的右端，从而表明T7-DNA右端的短片段在感染早期也能进入宿主细胞。
