Burbach J P
Neurosci Lett. 1983 Aug 8;38(3):281-5. doi: 10.1016/0304-3940(83)90382-8.
The biotransformation of [3H]des-Tyr1-gamma-endorphin locally in rat brain was studied by perfusion of the peptide via a push-pull cannula implanted in the nucleus caudatus. Metabolites were analyzed by high-pressure liquid chromatography. Free [3H]phenylalanine and a [3H]peptide with chromatographic properties of beta-endorphin2-13 were formed during push-pull perfusion. The method presented enables the detection of local proteolytic events in the brain and provides a novel tool for studies on neuropeptide biotransformation.
通过植入大鼠尾状核的推挽式套管灌注[3H]去酪氨酸1-γ-内啡肽，研究了其在大鼠脑内的局部生物转化。通过高压液相色谱分析代谢产物。在推挽式灌注过程中形成了游离的[3H]苯丙氨酸和具有β-内啡肽2-13色谱特性的[3H]肽。所提出的方法能够检测脑内局部蛋白水解事件，并为神经肽生物转化研究提供了一种新工具。
