[使用琼脂糖分离具有高免疫原性的流感病毒蛋白]
[Use of agarose for isolating influenza virus proteins retaining a high immunogenicity].
作者信息
Ivanova V T, Zakstel'skaia L Ia, Trushinskaia G N, Shenderovich S F, Liubovtseva O V
出版信息
Vopr Virusol. 1983 Jul-Aug;28(4):32-5.
PMID:6356601
Abstract
A method of influenza virus separation in agarose was modified. The possibility of using agarose of the national make and Difco agar was established. RNP and M proteins were isolated. Highly active anti-M and anti-RNP sera were prepared which work well in the ELISA and RIA.
摘要
一种在琼脂糖中分离流感病毒的方法得到了改进。确定了使用国产琼脂糖和Difco琼脂的可能性。分离出了核糖核蛋白（RNP）和基质蛋白（M蛋白）。制备了高效的抗M蛋白和抗RNP血清，它们在酶联免疫吸附测定（ELISA）和放射免疫分析（RIA）中效果良好。
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