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[利用固相放射免疫分析法对乙型流感病毒内部蛋白进行的演示与研究]

[Demonstration and study of the internal proteins of the influenza B virus using solid-phase radioimmunological analysis].

作者信息

Ivanova V T, Trushinskaia G N, Isachenko V A, Liubovtseva O V, Zakstel'skaia L Ia

出版信息

Vopr Virusol. 1985 Jul-Aug;30(4):394-7.

PMID:4060697
Abstract

Internal proteins of influenza B/USSR/14/80 virus retaining their antigenic and immunogenic activities were obtained by electrophoresis in 1% agarose. Antisera to eluted NP and M polypeptides were prepared. Study of influenza B viruses isolated in 1940-1984, performed by solid-phase radioimmunoassay, revealed no differences in the antigenic properties of nucleoprotein and matrix protein, except in the B/Yamagata/73 virus.

摘要

通过在1%琼脂糖中进行电泳,获得了保留其抗原和免疫活性的乙型流感病毒/苏联/14/80株的内部蛋白。制备了针对洗脱的核蛋白(NP)和基质蛋白(M)多肽的抗血清。通过固相放射免疫测定法对1940年至1984年分离的乙型流感病毒进行研究,结果显示,除了乙型/山形/73株病毒外,核蛋白和基质蛋白的抗原特性没有差异。

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