Steblay R W, Rudofsky U H
Am J Pathol. 1983 Nov;113(2):125-33.
The effects of injecting human, rabbit, rat, or single-kidney homologous glomerular basement membrane (GBM) or autologous GBM, each in complete Freund's adjuvant (CFA), into 15- to 18-month-old sheep are compared. All sheep receiving heterologous GBM and 3 of 6 sheep receiving homologous GBM had anti-GBM nephritis, but such sheep did not bind autoantibodies or have Goodpasturelike lesions in their lungs. Sheep given injections of human GBM had autoantibodies to antigenic determinants shared by fetal or adult sheep and human GBM, by lung basement membranes, and by certain nonvascular basement membranes. Sheep given homologous GBM had two populations of autoantibodies: one was neither species- nor organ-specific; the other was sheep-specific. No sheep given autologous GBM had any evidence of anti-GBM autoantibodies or nephritis. Their kidneys were indistinguishable by histologic, immunohistologic, and functional studies from CFA-treated controls. Thus, sheep seem very tolerant to autologous GBM. These findings suggest that human anti-GBM nephritis may occur if the GBM is altered so that it becomes cross-reacting and induces autoantibodies, as does homologous GBM.
比较了将人、兔、大鼠或单肾同源肾小球基底膜（GBM）或自体GBM分别与完全弗氏佐剂（CFA）一起注射到15至18月龄绵羊体内的效果。所有接受异源GBM的绵羊以及6只接受同源GBM的绵羊中的3只都发生了抗GBM肾炎，但这些绵羊并未结合自身抗体，肺部也没有出现类Goodpasture病变。注射人GBM的绵羊产生了针对胎儿或成年绵羊与人GBM、肺基底膜以及某些非血管基底膜共有的抗原决定簇的自身抗体。注射同源GBM的绵羊有两类自身抗体：一类既不具有物种特异性也不具有器官特异性；另一类是绵羊特异性的。没有一只注射自体GBM的绵羊有抗GBM自身抗体或肾炎的任何证据。通过组织学、免疫组织学和功能研究，它们的肾脏与接受CFA治疗的对照绵羊无法区分。因此，绵羊似乎对自体GBM具有很强的耐受性。这些发现表明，如果GBM发生改变，使其变得具有交叉反应性并诱导自身抗体，就像同源GBM那样，那么人类可能会发生抗GBM肾炎。
