Guisantes J A, Vicente F
Boll Ist Sieroter Milan. 1983 Mar 31;62(1):85-90.
The employment of Echinococcus granulosus protoscoleces embedded in paraffin as antigen for the indirect immunofluorescence (IIF) test for human hydatid disease is described. An evaluation of its sensitivity and specificity is made studying 108 pre-operative sera from surgically confirmed hydatidosis and 91 control sera. Using the positivity criterion based on the serological reactivity at which minimum cross-reactivity was observed, the IIF test showed an overall sensitivity of 83.3% and 2.2% of cross-reactions. No differences in sensitivity between sera from hepatic and pulmonary hydatidosis was observed. The advantages of the method are pointed out.
本文描述了将包埋在石蜡中的细粒棘球绦虫原头节用作抗原，用于人类包虫病间接免疫荧光（IIF）试验。通过研究108份手术确诊包虫病患者的术前血清和91份对照血清，对其敏感性和特异性进行了评估。采用基于观察到最小交叉反应的血清学反应性的阳性标准，IIF试验显示总体敏感性为83.3%，交叉反应率为2.2%。未观察到肝包虫病和肺包虫病血清在敏感性上的差异。文中指出了该方法的优点。
