Alekseenko L P, Zolotov N N, Pozdnev V F, Orekhovich V N
Biull Eksp Biol Med. 1983 Nov;96(11):29-32.
The paper is concerned with the distribution of prolylendopeptidase activity as measured by the hydrolysis of Z-Gly-Pro-pNA in extracts of various cattle tissues (hypophysis, liver, kidneys, heart, skeletal muscle, lungs, spleen, testicles, pancreas, thyroid, brain, small intestine, blood plasma). The highest specific activity was seen in the cerebral cortex, the lowest, in the spleen. The molecular weights of proteins with prolylendopeptidase activity in the extracts of the adenohypophysis, liver, kidneys, skeletal muscle and spleen were determined by gel filtration in an Ultrogel AcA 44 column, and the values of 57 000-66 000 dalton were obtained. Studies of the action of various inhibitors showed that complete inhibition of activity by PCMB (1 X 10(-3) M) was attained only in adenohypophyseal extracts, whereas in those of the spleen, skeletal muscle and liver, the inhibition reached 85%. The activity in the kidneys was inhibited by 65%.
本文关注的是通过测定Z-甘氨酰-脯氨酰-对硝基苯胺在各种牛组织（垂体、肝脏、肾脏、心脏、骨骼肌、肺、脾脏、睾丸、胰腺、甲状腺、大脑、小肠、血浆）提取物中的水解情况来研究脯氨酰内肽酶活性的分布。在大脑皮层中观察到最高的比活性，在脾脏中最低。通过在Ultrogel AcA 44柱上进行凝胶过滤，测定了腺垂体、肝脏、肾脏、骨骼肌和脾脏提取物中具有脯氨酰内肽酶活性的蛋白质的分子量，得到的值为57000 - 66000道尔顿。对各种抑制剂作用的研究表明，只有在腺垂体提取物中，PCMB（1×10⁻³ M）才能完全抑制活性，而在脾脏、骨骼肌和肝脏的提取物中，抑制率达到85%。肾脏中的活性被抑制了65%。
