从牛脑中分离脯氨酰内肽酶抑制肽。
Isolation of prolylendopeptidase-inhibiting peptides from bovine brain.
作者信息
Ohmori T, Nakagami T, Tanaka H, Maruyama S
机构信息
Department of Health and Dietary Science, Nippon Meat Packers, Inc., Ibaraki, Japan.
出版信息
Biochem Biophys Res Commun. 1994 Jul 29;202(2):809-15. doi: 10.1006/bbrc.1994.2002.
The peptides inhibiting prolylendopeptidase were screened and isolated from bovine brain by monitoring the inhibition of prolylendopeptidase produced by Flavobacterium meningosepticum. The amino acid sequence of the peptide having the highest inhibitory activity was determined as Met-Pro-Pro-Pro-Leu-Pro-Ala-Arg-Val-Asp-Phe-Ser-Leu-Ala-Gly-Ala-Leu-Asn. This peptide showed the IC50 and Ki values of 38.4 and 8.6 microM, respectively, for prolylendopeptidase isolated from bovine brain.
通过监测脑膜败血黄杆菌产生的脯氨酰内肽酶的抑制情况，从牛脑中筛选并分离出抑制脯氨酰内肽酶的肽。具有最高抑制活性的肽的氨基酸序列被确定为Met-Pro-Pro-Pro-Leu-Pro-Ala-Arg-Val-Asp-Phe-Ser-Leu-Ala-Gly-Ala-Leu-Asn。该肽对从牛脑中分离出的脯氨酰内肽酶的IC50和Ki值分别为38.4和8.6 microM。
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