Hambor J E, Fleck L, Stevenson J R
Cell Immunol. 1983 Oct 15;81(2):306-12. doi: 10.1016/0008-8749(83)90238-1.
Weanling CD2F1 mice were fed isocaloric diets that were protein sufficient (PS; containing 27% casein) or protein deficient (PD; containing 8% casein). Weight measurements demonstrated that the growth of PD mice was significantly impaired, thus indicating that the PD diet induced protein malnutrition. The cellular immune responsiveness of these mice was assessed from Day 21 to Day 49 of the diet using, as indicators, in vitro production of migration inhibitory factor (MIF) by splenic lymphocytes and MIF responsiveness of peritoneal macrophages. PD lymphocytes, when stimulated with the polyclonal activator concanavalin A, produced significantly less MIF than did PS lymphocytes. The amount of MIF produced by PD lymphocytes, however, increased throughout the study, possibly indicating delayed maturation of MIF synthetic capacity in PD mice. Normal CD2F1 mouse macrophages were used for these assays. MIF responsiveness of PD and PS macrophages was not significantly different when assayed using MIF produced by normal CD2F1 mouse lymphocytes. As compared to that of PS macrophages, the migratory ability of PD macrophages decreased progressively throughout the study. This impaired migratory ability did not interfere with MIF responsiveness of PD macrophages.
将断奶的CD2F1小鼠喂养于等热量饮食，这些饮食分为蛋白质充足组（PS；含27%酪蛋白）或蛋白质缺乏组（PD；含8%酪蛋白）。体重测量结果表明，PD组小鼠的生长显著受损，这表明PD饮食诱导了蛋白质营养不良。在饮食的第21天至第49天，以脾淋巴细胞体外产生迁移抑制因子（MIF）以及腹腔巨噬细胞对MIF的反应性为指标，评估这些小鼠的细胞免疫反应性。当用多克隆激活剂伴刀豆球蛋白A刺激时，PD组淋巴细胞产生的MIF明显少于PS组淋巴细胞。然而，在整个研究过程中，PD组淋巴细胞产生的MIF量有所增加，这可能表明PD组小鼠MIF合成能力的成熟延迟。这些实验使用的是正常CD2F1小鼠巨噬细胞。当用正常CD2F1小鼠淋巴细胞产生的MIF进行检测时，PD组和PS组巨噬细胞对MIF的反应性没有显著差异。与PS组巨噬细胞相比，在整个研究过程中，PD组巨噬细胞迁移能力逐渐下降。这种受损的迁移能力并未干扰PD组巨噬细胞对MIF反应性。
