Hauser H, Semenza G
CRC Crit Rev Biochem. 1983;14(4):319-45. doi: 10.3109/10409238309102798.
The isolation and purification of sucrase-isomaltase from brush border membrane is described and the physicochemical properties of the pure enzyme are discussed. Our present understanding of the mode of association of the intrinsic membrane protein sucrase-isomaltase with the brush border membrane will be the central point of this contribution. The assembly of sucrase-isomaltase into phospholipid bilayers has been reported to result in a model membrane system which resembles the "native" brush border membrane as regards the mode of lipid-protein interaction. The physicochemical properties of this reconstituted model membrane will be compared to the in vivo situation as represented by brush border membrane vesicles routinely isolated from small intestinal brush borders. The biosynthetic mechanism will be discussed.
本文描述了从刷状缘膜中分离和纯化蔗糖酶-异麦芽糖酶的方法，并讨论了纯酶的物理化学性质。我们目前对内在膜蛋白蔗糖酶-异麦芽糖酶与刷状缘膜结合方式的理解将是本文的核心内容。据报道，蔗糖酶-异麦芽糖酶组装到磷脂双层中会形成一个模型膜系统，该系统在脂质-蛋白质相互作用方式方面类似于“天然”刷状缘膜。将把这种重构模型膜的物理化学性质与从小肠刷状缘常规分离的刷状缘膜囊泡所代表的体内情况进行比较。还将讨论生物合成机制。
