Bobbioni E, Jeanrenaud B
Endocrinology. 1983 Dec;113(6):1958-62. doi: 10.1210/endo-113-6-1958.
Fractions from a partially purified ventrolateral hypothalamic (VLH) extract stimulate insulin (IRI) secretion when infused into isolated perfused rat pancreases. At a low glucose concentration (5 mM) in the perfusion medium, infusion of the VLH extract significantly increased insulin output when compared to controls infused with a cerebellar cortex extract. (IRI output with cerebellar cortex: 13.5 +/- 0.81; with VLH extracts: 40.9 +/- 9.4 ng/5 min, P less than 0.05). At a high glucose concentration (10 mM) in the perfusion medium, IRI secretion evoked by glucose was further augmented by the simultaneous infusion of the VLH extract (IRI output with glucose: 325.6 +/- 25.8; glucose + VLH extract: 448.1 +/- 33.0 ng/20 min, P less than 0.02). When pancreases were perfused with a solution containing 20 different amino acids [(AA) 6.6 mM final concentration] IRI secretion elicited by the AA was similarly augmented by the presence of the VLH extract (IRI output with AA: 101.8 +/- 14.9; AA + VLH extract: 179.8 +/- 21.9, P less than 0.02). However, IRI secretion as stimulated by arginine alone (3 mM) was not potentiated by the extract. It is suggested that the hypothalamic factor(s) responsible for this IRI secretion-promoting activity could contribute to the hypothalamic control of IRI secretion through a route which remains to be established.
将部分纯化的下丘脑腹外侧核（VLH）提取物注入离体灌注的大鼠胰腺时，其组分可刺激胰岛素（IRI）分泌。在灌注液中葡萄糖浓度较低（5 mM）时，与注入小脑皮质提取物的对照组相比，注入VLH提取物可显著增加胰岛素分泌量。（注入小脑皮质提取物后的IRI分泌量：13.5±0.81；注入VLH提取物后的IRI分泌量：40.9±9.4 ng/5分钟，P<0.05）。在灌注液中葡萄糖浓度较高（10 mM）时，同时注入VLH提取物可使葡萄糖诱导的IRI分泌进一步增加（葡萄糖刺激后的IRI分泌量：325.6±25.8；葡萄糖+VLH提取物：448.1±33.0 ng/20分钟，P<0.02）。当用含有20种不同氨基酸（终浓度6.6 mM）的溶液灌注胰腺时，VLH提取物的存在同样可增加氨基酸诱导的IRI分泌（氨基酸刺激后的IRI分泌量：101.8±14.9；氨基酸+VLH提取物：179.8±21.9，P<0.02）。然而，提取物不能增强单独由精氨酸（3 mM）刺激引起的IRI分泌。提示负责这种促进IRI分泌活性的下丘脑因子可能通过一条尚待确定的途径参与下丘脑对IRI分泌的调控。
