Helman A M, Amira R, Nicolaïdis S, Assan R
Endocrinology. 1980 May;106(5):1612-9. doi: 10.1210/endo-106-5-1612.
Catheterization of the portal vein and stereotaxic implantation of electrodes in the ventrolateral hypothalamus (VLH) were performed in normal rats after thiopental anesthesia. Immunoreactive glucagon (IRG), insulin (IRI), And glucose were monitored in portal plasma before and during electrical stimulation of the VLH (6 micro A, 50 Hz, 2 msec each, for 15 min). This stimulation induced a significant and reproducible IRG rise, followed by hyperglycemia. IRI remained unchanged. These alterations were not observed in control rats, i.e. in the absence of implantation; after VLH implantation without stimulation; or after implantation in the hippocampus or in the nucleus lenticularis. Bilateral splanchnicectomy abolished the IRG rise and hyperglycemia which followed VLH stimulation, while IRI was elevated both before and during electrical stimulation. Bilateral vagotomy did not suppress the A cell response to VLH stimulation, and it significantly reduced the IRI concentration in both basal and stimulatory periods. This resulted in sustained hyperglycemia. Attempts at total denervation of the pancreas induced patterns similar to that observed after splanchnicectomy alone. These results suggest that stimulation of the VLH can influence the endocrine pancreas and blood glucose levels by sympathetic nervous inputs which stimulate A cells and inhibit B cells.
在硫喷妥钠麻醉后，对正常大鼠进行门静脉插管以及在腹外侧下丘脑（VLH）进行立体定向电极植入。在VLH电刺激前及刺激期间（6微安、50赫兹、每次2毫秒，持续15分钟），监测门静脉血浆中的免疫反应性胰高血糖素（IRG）、胰岛素（IRI）和葡萄糖。这种刺激导致IRG显著且可重复地升高，随后出现高血糖。IRI保持不变。在对照大鼠中未观察到这些变化，即未进行植入时；在植入VLH但未刺激时；或在海马体或豆状核植入后。双侧内脏神经切除术消除了VLH刺激后出现的IRG升高和高血糖，而在电刺激前及刺激期间IRI均升高。双侧迷走神经切断术并未抑制A细胞对VLH刺激的反应，且在基础期和刺激期均显著降低了IRI浓度。这导致持续的高血糖。对胰腺进行完全去神经支配的尝试所诱导的模式与单独进行内脏神经切除术后观察到的模式相似。这些结果表明，刺激VLH可通过刺激A细胞并抑制B细胞的交感神经输入来影响内分泌胰腺和血糖水平。
