Louvard D, Morris C, Warren G, Stanley K, Winkler F, Reggio H
EMBO J. 1983;2(10):1655-64. doi: 10.1002/j.1460-2075.1983.tb01640.x.
Monoclonal antibodies have been raised to pig brain triskelions and one clone, DC41, was found to recognize the clathrin heavy chain by immunoblotting. However, both by immunofluorescence and immunoelectron microscopy, and in complete contrast to polyclonal anti-clathrin antibodies, monoclonal DC41 did not label either coated pits or coated vesicles anywhere in the cell. Instead it appeared to label the cell cytoplasm. These data suggest that DC41 recognizes a cytoplasmic form of clathrin, perhaps that form produced by uncoating of coated vesicles which is then ready to re-build another coated pit.
已经制备了针对猪脑三脚蛋白的单克隆抗体，通过免疫印迹发现其中一个克隆DC41能识别网格蛋白重链。然而，无论是通过免疫荧光和免疫电子显微镜观察，还是与多克隆抗网格蛋白抗体完全相反的是，单克隆抗体DC41在细胞内的任何位置都没有标记包被小窝或包被小泡。相反，它似乎标记了细胞质。这些数据表明，DC41识别的是细胞质形式的网格蛋白，可能是包被小泡脱包被后产生的那种形式，然后准备重新形成另一个包被小窝。
