Barque J P, Danon F, Peraudeau L, Yeni P, Larsen C J
EMBO J. 1983;2(5):743-9. doi: 10.1002/j.1460-2075.1983.tb01494.x.
Using a serum from a patient with an autoimmune disease, we have recently described a novel 55 000-dalton antigen (p55) in the nucleus of several animal cells including human ones. This antigen, designated PSL, was not related to the previously defined antigens recognized by sera from patients with systemic rheumatic diseases (Sm, n-RNP, SS-B, Scl-70). We have now found that p55 is associated with chromatin structures as it is released from the nucleus of mink cell fibroblasts by saline + DNase treatments. Analysis by sucrose gradient centrifugation of the nuclear material released in these conditions indicated that p55 co-migrated with core histones. Meanwhile, p55 was absent from the residual nuclear matrices (achromatinic nuclei). Localization of p55 in synchronized cells was performed by indirect immunofluorescence and immunoprecipitation. P55 appeared to accumulate in the nucleus during the S phase. Finally, it was not recognized by an anti-SV40 tumor serum that specifically precipitated the protein p53, which has been recently related to cell proliferation. Thus, PSL an p53, although apparently not antigenically related, appear to be implicated in the same step of the cell cycle.
利用一名自身免疫性疾病患者的血清，我们最近在包括人类细胞在内的多种动物细胞的细胞核中发现了一种新的55000道尔顿抗原（p55）。这种抗原被命名为PSL，与系统性风湿性疾病患者血清所识别的先前定义的抗原（Sm、n-RNP、SS-B、Scl-70）无关。我们现在发现，p55与染色质结构相关，因为通过盐水+DNase处理，它会从貂肺细胞成纤维细胞核中释放出来。对在这些条件下释放的核物质进行蔗糖梯度离心分析表明，p55与核心组蛋白共迁移。同时，在残留的核基质（无染色质核）中不存在p55。通过间接免疫荧光和免疫沉淀法对同步化细胞中的p55进行定位。P55似乎在S期在细胞核中积累。最后，它不被能特异性沉淀最近与细胞增殖相关的蛋白p53的抗SV40肿瘤血清所识别。因此，PSL和p53虽然显然没有抗原相关性，但似乎在细胞周期的同一阶段发挥作用。
