Immunocytochemical demonstration of Con A-receptors on the cell surface of guinea pig peritoneal macrophages.

By means of protein A-gold in combination with the replica technique the distribution of Con A-receptors on the surface of guinea pig peritoneal macrophages was investigated. In nonspecifically activated macrophages the arrangement of Con A-binding sites was demonstrated as homogeneous. It could be evidenced that by the combined technique the advantages of the high resolution of transmission electron microscopy could be joined with the possibility of the stereological demonstration of cell surface structures.