Peschke T, Gabert A, Wollweber L, Augsten K
Acta Histochem Suppl. 1986;33:201-6.
The influence of various fixatives (glutaraldehyde, formaldehyde, OsO4) on the retention of antigenicity of peritoneal macrophages was studied by means of fluorescence microscopy, scanning electron microscopy, and X-ray microanalysis. After fixation with formaldehyde the Concanavalin A (Con A) binding sites were found to be highest, whereas the fixation with OsO4 results in loss of many binding sites. The fluorescence microscopic investigations of glutaraldehyde fixed cells is impaired by fixative induced fluorescence. Therefore X-ray microanalytical determinations show better results for these cells than fluorescence microscopic measurements. On contrary to direct labelling method the number of unspecific Con A binding sites on peritoneal macrophages labelled with the sandwich method is negligible.
通过荧光显微镜、扫描电子显微镜和X射线微分析研究了各种固定剂（戊二醛、甲醛、四氧化锇）对腹膜巨噬细胞抗原性保留的影响。用甲醛固定后，发现刀豆球蛋白A（Con A）结合位点最高，而用四氧化锇固定会导致许多结合位点丧失。戊二醛固定细胞的荧光显微镜研究受到固定剂诱导荧光的影响。因此，对于这些细胞，X射线微分析测定比荧光显微镜测量显示出更好的结果。与直接标记法相反，用夹心法标记的腹膜巨噬细胞上非特异性Con A结合位点的数量可以忽略不计。
