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Interference microscopic determination of the section thickness of different paraffin-embedded organ tissues.

作者信息

Stiller K J, Süss B

出版信息

Exp Pathol. 1983;24(2-3):213-5. doi: 10.1016/s0232-1513(83)80035-8.

DOI:10.1016/s0232-1513(83)80035-8
PMID:6357835
Abstract

With respect to the significance of the section thickness in quantitative-morphological studies the thickness of different paraffin-embedded tissue sections was determined by interference microscopy. Within the range of 3-5 microns the measurements revealed accordance of the measured section thickness with that adjusted on the sliding microtome. In the range surpassing 5 microns the section thickness obtained was in all cases lower than that adjusted on the microtome. The deviations were up to 40%.

摘要

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