Yoh M, Yamamoto K, Honda T, Takeda Y, Miwatani T
Infect Immun. 1983 Nov;42(2):778-82. doi: 10.1128/iai.42.2.778-782.1983.
Enterotoxigenic Escherichia coli grown in the presence of lincomycin and tetracycline produced an increased amount of heat-labile enterotoxin (LT). These antibiotics increased the production of not only extracellular LT but also intracellular LT. On the other hand, lincomycin did not stimulate the production of heat-stable enterotoxin by enterotoxigenic E. coli. The extracellular LTs produced in the presence of lincomycin and tetracycline were purified and analyzed by sodium dodecyl sulfate-polyacrylamide gel disc electrophoresis. Results showed that the A subunits of the purified LTs were not nicked, unlike that of extracellular LT produced in the absence of the antibiotics.
在林可霉素和四环素存在的情况下生长的产肠毒素大肠杆菌产生了更多的热不稳定肠毒素（LT）。这些抗生素不仅增加了细胞外LT的产量，也增加了细胞内LT的产量。另一方面，林可霉素不会刺激产肠毒素大肠杆菌产生热稳定肠毒素。对在林可霉素和四环素存在的情况下产生的细胞外LT进行纯化，并通过十二烷基硫酸钠-聚丙烯酰胺凝胶圆盘电泳进行分析。结果表明，与在无抗生素情况下产生的细胞外LT不同，纯化后的LT的A亚基没有切口。
