Martin F, Caignard A, Jeannin J F, Leclerc A, Martin M
Int J Cancer. 1983 Nov 15;32(5):623-7. doi: 10.1002/ijc.2910320517.
From an established cell culture line obtained from a chemically-induced rat colon carcinoma, two sublines have been selected and isolated according to their susceptibility to trypsin-mediated detachment from plastic surfaces. Subline TR, the most resistant to the detaching effect of trypsin, gave progressive tumors in most of the syngeneic rats in which it was inoculated. Subline TS, which was easily detached by trypsin, also gave tumors in the syngeneic rats, but all these tumors disappeared within 3 or 4 weeks. Both sublines gave progressive tumors when injected into nude mice. This suggests that the parent cell line is heterogeneous and contains cell variants differing in their susceptibility to trypsin-mediated detachment from substrate and their sensitivity to host factors leading to acceptance or rejection of the inoculated tumor cells.
从一株通过化学诱导产生的大鼠结肠癌建立的细胞系中，根据对胰蛋白酶介导的从塑料表面脱离的敏感性，选择并分离出了两个亚系。亚系TR对胰蛋白酶的脱离作用最具抗性，在接种了它的大多数同基因大鼠中产生了进行性肿瘤。亚系TS很容易被胰蛋白酶分离，在同基因大鼠中也产生了肿瘤，但所有这些肿瘤在3或4周内消失。当注入裸鼠体内时，两个亚系都产生了进行性肿瘤。这表明亲代细胞系是异质的，包含细胞变体，这些变体在对胰蛋白酶介导的从底物脱离的敏感性以及对导致接种肿瘤细胞被接受或排斥的宿主因子的敏感性方面存在差异。
