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应用扫描细胞光度法对免疫细胞化学制备的幼年虹鳟垂体促性腺激素进行定量估计。

Quantitative estimation of pituitary gonadotropin in juvenile rainbow trout with scanning cytophotometry applied to immunocytochemical preparations.

作者信息

Terlou M, van den Hurk R, Gielen J T

出版信息

Histochemistry. 1983;79(2):237-44. doi: 10.1007/BF00489785.

DOI:10.1007/BF00489785
PMID:6358148
Abstract

A densitometrical method with computer-controlled scanning cytophotometry has been developed to quantify the amount of gonadotropin (GTH) in immunocytochemically treated paraffin sections of pituitaries of juvenile rainbow trout. Results obtained with this method totally correspond with those established with a radioimmunoassay of pooled pituitaries. Scanningcytophotometry offers the advantage that GTH-content of individual pituitaries of juvenile or small adult fish can be investigated.

摘要

已开发出一种采用计算机控制扫描细胞光度测定法的密度测定方法，用于定量幼年虹鳟垂体免疫细胞化学处理石蜡切片中的促性腺激素（GTH）含量。用该方法获得的结果与通过对合并垂体进行放射免疫测定所确定的结果完全一致。扫描细胞光度测定法的优点是可以研究幼年或成年小鱼个体垂体的GTH含量。

相似文献

1
Quantitative estimation of pituitary gonadotropin in juvenile rainbow trout with scanning cytophotometry applied to immunocytochemical preparations.应用扫描细胞光度法对免疫细胞化学制备的幼年虹鳟垂体促性腺激素进行定量估计。
Histochemistry. 1983;79(2):237-44. doi: 10.1007/BF00489785.
2
Accumulation of glycoprotein gonadotropin in the pituitary of juvenile rainbow trout in response to androgens and C21-steroids, including 11-steroids.幼年虹鳟垂体中糖蛋白促性腺激素因雄激素和C21类固醇（包括11-类固醇）作用而积累。
Cell Tissue Res. 1984;235(3):635-42. doi: 10.1007/BF00226962.
3
Salmonid pituitary gonadotrophs. I. Distinct cellular distributions of two gonadotropins, GTH I and GTH II.鲑鱼垂体促性腺激素细胞。I. 两种促性腺激素GTH I和GTH II的不同细胞分布。
Gen Comp Endocrinol. 1990 Mar;77(3):348-57. doi: 10.1016/0016-6480(90)90224-a.
4
Salmonid pituitary gonadotrophs. II. Ontogeny of GTH I and GTH II cells in the rainbow trout (Salmo gairdneri irideus).鲑科鱼类垂体促性腺激素细胞。II. 虹鳟（Salmo gairdneri irideus）中促性腺激素I和促性腺激素II细胞的个体发生。
Gen Comp Endocrinol. 1990 Mar;77(3):358-67. doi: 10.1016/0016-6480(90)90225-b.
5
Seasonal variations in plasma and pituitary levels of gonadotrophin in males and females of two strains of rainbow trout (Salmo gairdneri).两种虹鳟鱼（萨氏虹鳟）雌雄个体血浆和垂体中促性腺激素水平的季节性变化。
Gen Comp Endocrinol. 1989 Sep;75(3):376-88. doi: 10.1016/0016-6480(89)90172-x.
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Effect of 17 alpha-methyltestosterone, estradiol-17 beta and synthetic LHRH on production of gonadotropic hormone in pituitaries of rainbow trout (organ culture).17α-甲基睾酮、17β-雌二醇和合成促性腺激素释放激素对虹鳟垂体促性腺激素分泌的影响（器官培养）
Cell Tissue Res. 1983;232(1):157-76. doi: 10.1007/BF00222381.
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Immunoreactive gonadotropin-releasing hormone-like material in the brain and the pituitary gland during the periovulatory period in the brown trout (Salmo trutta L.): relationships with the plasma and pituitary gonadotropin.褐鳟（Salmo trutta L.）排卵期前后大脑和垂体中免疫反应性促性腺激素释放激素样物质：与血浆和垂体促性腺激素的关系
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The brain-pituitary-gonadal axis in the rainbow trout, Salmo gairdneri. II. Direct effect of gonadal steroids on the gonadotropic cells.虹鳟鱼（Salmo gairdneri）的脑-垂体-性腺轴。II. 性腺类固醇对促性腺细胞的直接作用。
Cell Tissue Res. 1983;233(2):377-88. doi: 10.1007/BF00238304.
9
Manipulation of the hypothalamo-pituitary gonadal axis in the juvenile rainbow trout: influence of pituitary transplantation and LHRH analogue treatment.幼年虹鳟下丘脑-垂体-性腺轴的调控：垂体移植和促性腺激素释放激素类似物处理的影响
Reprod Nutr Dev (1980). 1985;25(5):895-907. doi: 10.1051/rnd:19850706.
10
Gonadotropin stimulation of estrogen and yolk precursor synthesis in juvenile rainbow trout.促性腺激素对幼年虹鳟鱼雌激素和卵黄前体合成的刺激作用。
Gen Comp Endocrinol. 1980 Jul;41(3):384-91. doi: 10.1016/0016-6480(80)90083-0.

引用本文的文献

1
Accumulation of glycoprotein gonadotropin in the pituitary of juvenile rainbow trout in response to androgens and C21-steroids, including 11-steroids.幼年虹鳟垂体中糖蛋白促性腺激素因雄激素和C21类固醇（包括11-类固醇）作用而积累。
Cell Tissue Res. 1984;235(3):635-42. doi: 10.1007/BF00226962.

本文引用的文献

1
The brain-pituitary-gonadal axis in the rainbow trout, Salmo gairdneri: gonadal hormones and the maturation of gonadotropic cells.虹鳟鱼（Salmo gairdneri）的脑-垂体-性腺轴：性腺激素与促性腺细胞的成熟
Cell Tissue Res. 1982;225(1):45-56. doi: 10.1007/BF00216217.
2
Effects of steroids on gonadotropic (GTH) cells in the pituitary of rainbow trout, Salmo gairdneri, shortly after hatching.孵化后不久，类固醇对虹鳟（Salmo gairdneri）垂体中促性腺激素（GTH）细胞的影响。
Cell Tissue Res. 1982;224(2):361-8. doi: 10.1007/BF00216879.
3
Accumulation of glycoprotein gonadotropin in the pituitary of juvenile rainbow trout in response to androgens and C21-steroids, including 11-steroids.
幼年虹鳟垂体中糖蛋白促性腺激素因雄激素和C21类固醇（包括11-类固醇）作用而积累。
Cell Tissue Res. 1984;235(3):635-42. doi: 10.1007/BF00226962.
4
The brain-pituitary-gonadal axis in the rainbow trout, Salmo gairdneri. II. Direct effect of gonadal steroids on the gonadotropic cells.虹鳟鱼（Salmo gairdneri）的脑-垂体-性腺轴。II. 性腺类固醇对促性腺细胞的直接作用。
Cell Tissue Res. 1983;233(2):377-88. doi: 10.1007/BF00238304.
5
Steroidogenesis in the gonads of rainbow trout fry (Salmo gairdneri) before and after the onset of gonadal sex differentiation.虹鳟鱼苗（萨氏虹鳟）性腺性别分化开始前后性腺中的类固醇生成。
Reprod Nutr Dev (1980). 1982;22(2):413-25. doi: 10.1051/rnd:19820313.
6
Densitometric measurement of two cellular parameters related to the secretory activity of the subcommissural organ.与连合下器官分泌活动相关的两个细胞参数的密度测量。
Microsc Acta. 1981 Sep;85(1):25-44.
7
Estradiol-positive feedback on gonadotropic (GTH) cells in freshwater male silver eels.淡水雄性银鳗中雌二醇对促性腺激素（GTH）细胞的正反馈作用。
Gen Comp Endocrinol. 1979 Nov;39(3):247-61. doi: 10.1016/0016-6480(79)90119-9.
8
A useful technique applicable to the immunofluorescence test.一种适用于免疫荧光检测的有用技术。
J Immunol Methods. 1977;18(3-4):391-2. doi: 10.1016/0022-1759(77)90194-6.