酿酒酵母中六种（1→3）-β-葡聚糖酶的分离与鉴定
Separation and characterization of six (1 leads to 3)-beta-glucanases from Saccharomyces cerevisiae.
作者信息
Hien N H, Fleet G H
出版信息
J Bacteriol. 1983 Dec;156(3):1204-13. doi: 10.1128/jb.156.3.1204-1213.1983.
Abstract
Using a system of chromatography through columns of DEAE-Bio-Gel, HTP-Bio-Gel, and CM-Bio-Gel, we isolated and characterized six different (1 leads to 3)-beta-glucanases from cell wall autolysates and cell extracts of Saccharomyces cerevisiae haploid strain 2180B. These enzymes were designated glucanases I, II, IIIA, IIIB, IV, and V. The haploid mating type S. cerevisiae strain 2180A and the diploid strains S. cerevisiae 2180D and S. cerevisiae 595 contained the same complex of glucanases. Glucanases II and IIIA were exoenzymes, and glucanases I, IIIB, IV, and V were endoenzymes. The enzymes exhibited different molecular weights, kinetic properties, and activities on isolated yeast cell walls. The products of substrate (laminarin) hydrolysis were quantified by using high-pressure liquid chromatography and were significantly different for the four endoglucanases.
摘要
我们使用通过DEAE-生物凝胶柱、HTP-生物凝胶柱和CM-生物凝胶柱的色谱系统，从酿酒酵母单倍体菌株2180B的细胞壁自溶物和细胞提取物中分离并鉴定了六种不同的（1→3）-β-葡聚糖酶。这些酶被命名为葡聚糖酶I、II、IIIA、IIIB、IV和V。酿酒酵母单倍体交配型菌株2180A以及二倍体菌株酿酒酵母2180D和酿酒酵母595含有相同的葡聚糖酶复合物。葡聚糖酶II和IIIA是外切酶，而葡聚糖酶I、IIIB、IV和V是内切酶。这些酶在分离的酵母细胞壁上表现出不同的分子量、动力学特性和活性。通过高压液相色谱对底物（海带多糖）水解产物进行定量，四种内切葡聚糖酶的水解产物有显著差异。
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