pRD1向丁香假单胞菌的转移及其整合到染色体中的证据。
Transfer of pRD1 to Pseudomonas syringae and evidence for its integration into the chromosome.
作者信息
Vincent J R, Fulbright D W
出版信息
J Bacteriol. 1983 Dec;156(3):1349-51. doi: 10.1128/jb.156.3.1349-1351.1983.
Abstract
Plasmid pRD1 was conjugatively transferred from Escherichia coli to Pseudomonas syringae. Subculturing the transconjugate on a medium that selected for pRD1-determined His+ Kmr resulted in the loss of pRD1 as an extrachromosomal element as detected by agarose gel electrophoresis. DNA hybridization provided evidence for the integration of pRD1 into the P. syringae chromosome.
摘要
质粒pRD1通过接合作用从大肠杆菌转移至丁香假单胞菌。将接合子在选择pRD1决定的His⁺ Kmr的培养基上进行传代培养，结果如琼脂糖凝胶电泳检测所示，pRD1作为一种染色体外元件丢失。DNA杂交为pRD1整合到丁香假单胞菌染色体中提供了证据。
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